#Cellprofiler identify chromosomes manuals
#Cellprofiler identify chromosomes plus

#Cellprofiler identify chromosomes plus

In vivo the plus end of a microtubule is distal to the microtubule organizing center. It has therefore become the convention to call the rapidly polymerizing end the plus-end of a microtubule and the slowly polymerizing end the minus-end. Tubulin can polymerize from both ends in vitro, however, the rate of polymerization is not equal.

Microtubules are highly ordered fibers that have an intrinsic polarity, shown schematically in figure A. When tubulin polymerizes it initially forms protofilaments, microtubules consist of 13 protofilaments and are 25 nm in diameter, each µm of microtubule length being composed of 1650 heterodimers. Tubulin polymerizes to form structures called microtubules (MTs). Tubulin heterodimers polymerize to form microtubules. However, in pharmacological terms these minor differences can be used to enhance the specificity of drugs for certain diseasse (6). In biochemical terms, this fact results in the technical benefit that porcine tubulin (in the form of microtubules, see below) can be used to assay tubulin associated proteins originating from many diverse species, e.g.: yeast (1,2,3) and fruit fly (4,5). Consequently, tubulin isolated from porcine brain tissue is highly homologous to tubulin isolated from any eukaryotic source. These two proteins are encoded by separate genes, or small gene families, whose sequences are highly conserved throughout the eukaryotic kingdom. Tubulin is composed of a heterodimer of two closely related 55 kDa proteins called α and β tubulin.

#Cellprofiler identify chromosomes manuals

More detailed information is described in the manuals of individual Tubulin products and tubulin based Biochem™ Kits.

Below we have outlined some of the basic priniciples involved in microtubule formation and how you can manipulate experimental conditions according to your needs. This section is intended for those researchers who are not accustomed to working with Tubulin protein in vitro.

MemGlow™ Plasma Membrane Probes for Bioimaging 8.

Small G-protein Activators & Inhibitors 5.

GOBlot - The First Affordable Western Blot Processor.

New Signal-Seeker™: PTM detection kits, antibodies, and reagents.